A study on the relationship between social support and life satisfaction among patients with irritable bowel syndrome and the effect of self -esteem as a mediator / 预防医学

Objective To analyze the mediating effect of self -esteem on the relationship between social support and life satisfaction among patients with irritable bowel syndrome (IBS).Methods A total of 197 IBS patients were evaluated using questionnaires of life satisfaction scale,self -esteem scale and perceived social support scale.Results The effects of gender and education on life satisfaction of IBS patients were not significant (P >0.05 ).Life satisfaction was significantly correlated with social support and self -esteem (|r|=0.321 -0.388,P <0.01).Social support was not only had direct effect on life satisfaction (β=0.53,P <0.01 ),but also had indirect effect through self -esteem on life satisfaction (β=0.09,P <0.01).Conclusion Life satisfaction of patients with IBS was influenced by social support and self -esteem,and self -esteem played the partial intermediary role in the influencing model.

Research on the association between per-capita tobacco consumption among the permanent residents and lung cancer mortality in Henan province / 中华流行病学杂志

Objective To study the relationship of per-capita tobacco consumption and lung cancer mortality in Henan province,and to provide evidence for policy development on tobacco control and reduction of lung cancer mortality.Methods Data regarding lung cancer mortality and per-capita tobacco consumption among household residents from 1992 to 2011,was collected from published almanacs in Henan and Henan Tumor Institutes.Trend Method was used to analyze the development of lung cancer in Henan province and the trend of per-capita tobacco consumption of residents in the household.‘Spearman rank correlation' was used to analyze the correlation between per-capita tobacco consumption of residents in the household from 1992 to 2001 and the lung cancer mortality rates from 2002 to 2011,with the lag time unite as 10 years in this study.Cure Estimation was used to fit the model regarding the relationship between per-capita tobacco consumption of residents in the household and lung cancer.Results Lung cancer mortality among those permanent residents in Henan province increased from 14.75/100 000 in 1992 to 27.00/100 000 in 2011,with an increase of 83.05％.Both the trend of per-capita tobacco consumption among the permanent residents and the lung cancer mortality were uprising,with the tobacco consumption showing a lag effect to the lung cancer mortality.Correlation coefficient between the per-capita tobacco consumption of residents in the household from 1992 to 2001 and the lung cancer mortality from 2002 to 2011 was rs=0.770,P=0.009＜0.05,with statistically significant difference.Along with the uprising trend of lung cancer mortality,the per-capita tobacco consumption of residents in the household was also parallelly rising with the equation of relevance betweenper-capita tobacco consumption of residents in the household in Henna province and lung cancer as y =2.60 x0.46 (F=576.483) and the R2 was 0.667.Conclusion Per-capita tobacco consumption of residents in the household in Henan province appeared a factor that influencing the lung cancer mortality and an association between the per-capita tobacco consumption of residents in the household and lung cancer was noticed.Tobacco consumption had a lag trend to the mortality of lung cancer.

Correlation of the methylation status of CpG islands in the promoter region of 10 genes with the 5-Fu chemosensitivity in 3 breast cancer cell lines / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To explore the relationship between the methylation status of CpG islands in the promoter region of 10 genes in breast cancer cells and their sensitivity to 5-fluouracil (5-Fu), and to identify the genes responsible for the 5-Fu resistance in breast cancer.</p><p><b>METHODS</b>Three cell lines (differently resistant to chemotherapy) were used in this study: Bcap-37 (IC(50): 289.77 microg/ml), T47D (IC(50): 134.16 microg/ml) and ZR-75-30 (IC(50): 4.20 microg/ml). The methylation profile of 10 genes (BAG1, C11ORF31, CBR1, CBR4, GJA1, FOXL2, IGFBP6, P4HA1, SRI and TYMS) in the 3 breast cancer cell lines was determined by methylation specific PCR. The steady-state mRNAs of ABCC8, CHFR and IGFBP6 genes were quantified by real-time RT PCR analysis.</p><p><b>RESULTS</b>Among the 10 genes, only genes IGFBP6 and FOXL2 displayed differential DNA methylation pattern between the 5-Fu-resistant and 5-Fu-sensitive cell lines. The mRNA expression level of genes PRSS21, LOX, IGFBP6, ABCC8 and CHFR was quantified by real-time RT-PCR analysis. Except for CHFR, the expression level of the other 4 genes was correlated with the methylation status of CpG islands, namely, a lower expression level with methylation status and a higher level with demethylation status.</p><p><b>CONCLUSION</b>The results of the present study have demonstrated that there are 8 genes with differential methylation status in chemosensitive and chemoresistant breast cancer cell lines, i.e. two genes more than the six genes we reported previously. Our findings provide both mechanistic insights for the drug resistance of breast cancer and the basis for further studies on potential application of the DNA methylation in this set of genes for prediction of chemosensitivity of breast cancer.</p>